Hippocampal microcircuits are comprised of excitatory pyramidal cells, which integrate information and innervate downstream brain regions, and inhibitory interneurons, which function locally to regulate pyramidal cell activity and synchronicity. In the ventral hippocampus (vHipp), microcircuit dysfunction has been associated with a variety of neurological disorders, including neurodegenerative diseases, neurodevelopmental disorders, and psychiatric illnesses. Previous work has demonstrated that vHipp pyramidal cells differentially regulate schizophrenia-like behaviors depending on their downstream target. Similarly, unique classes of inhibitory interneurons (namely parvalbumin (PV)-positive and somatostatin(SST)-positive) also differently regulate schizophrenia-like behaviors. Therefore, the overarching hypothesis of the current proposal is that PV- and SST-positive interneurons differentially regulate the function of ventral hippocampus pyramidal cells depending on their projection target. Techniques such as mGRASP and fiber photometry will be used to determine if microcircuit anatomy and function are altered by chronic stress, a predisposing factor for many neurological disorders. The results will provide insight into basic principles of neural circuit function and may lead to new strategies for the treatment and prevention of devastating neurological disorders.
Motivated, dedicated, and reliable volunteers are desired. Previous research experience and biology or neuroscience coursework are considered a plus, but not required. Applicants will be expected to devote a minimum of 12 hours per week (including some weekend work) for at least 6 months.
Assist with animal husbandry
Assist with chronic stress procedures and behavioral testing
Assist with data collection and analysis
Additional duties according to volunteer's level of commitment and aptitude
